Identification of various T-2 toxin metabolites in chicken excreta and tissues

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Identification of various T-2 toxin metabolites in chicken excreta and tissues.

Gas chromatography-mass spectrometry was used to identify various T-2 toxin metabolites in chicken excreta and organs 18 h after intraperitoneal injection of the toxin. No trichothecenes were detected in the heart and kidneys, and only trace amounts were detected in the lungs. Most of the T-2 metabolites were found in the excreta, although considerable amounts were also found in the liver. In a...

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T-2 metabolites in the excreta of broiler chickens administered 3H-labeled T-2 toxin.

A method for the detection of T-2 metabolites was developed and applied to analysis of metabolites in excreta of broiler chickens administered 3H-labeled T-2 toxin. The method used acetonitrile extraction and partitioning with petroleum ether followed by chromatography on Amberlite XAD-2, Florisil, and Sep-Pak C18. The recovery of T-2 toxin added to the chicken excreta was 73% at a concentratio...

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Pathology of chlorpyriphos and T-2 toxin on broiler chicken

Forty-eight, newly hatched, unsexed broiler chicks were fed diets containing 45 ppm chlorpyriphos, an organophosphorus compound and 0.5 ppm T-2, a mycotoxin, singly and in combination for 28 days from day of hatch to study of pathological effects. Gross, pale, enlarged liver, distended gall bladder and streaks of haemorrhages in the thigh muscles were observed in the chlorpyriphos group, while ...

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Arsenic in Human Tissues and Excreta

Arsenic is an important poison bot 1 or homicidal and suicidal purposes. As it is w 1 e y distributed in nature exact information a oil the amount of arsenic normally present m human tissues, excreta and foodstuns is great importance in medico-legal inves1 iga 10 When it is present in large quantity in viscera of a suspected case of arsenic poisoning, no difficulty arises in giving a defini e o...

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Inhibition of mitogen-induced blastogenesis in human lymphocytes by T-2 toxin and its metabolites.

Concentrations of T-2, HT-2, 3'-OH T-2, 3'-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. The results suggested that the initial hydrolysis of T-2 toxin and the hydroxylation of T-2 toxin to 3'-OH T-2 toxin did not significantly decrease the immuno...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1985

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.49.5.1246-1250.1985